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Fluid Imaging

Operating Principles

Rev. D Dimension 3100 Manual 171

10.5.17 Engage

1. Select Motor > Engage. A pre-engage check begins, followed by Z-stage motor motion.

2. To move to another area of the sample, execute a Withdraw command to avoid damaging

the tip and scanner.

3. Select Stage > Focus Surface and move the stage using the trackball.

Note: After the tip is engaged, adjust the control panel values to provide the desired

scan parameters.

10.5.18 Adjust Scan Parameters

Once engaged, the scan parameters should be adjusted to obtain the best image. Set the Slow scan

axis to Disabled and select View > Scope Mode. Observe the agreement between the trace and

retrace lines into, and adjust the Setpoint, Gains and Scan rate to bring the trace and retrace lines

in to coincidence. This procedure will be similar to operation in air, with the following exceptions:

• Samples are generally softer in ﬂuids. Adjusting the applied forces becomes more

critical.

• Scan rates tend to be slower in ﬂuid.

Adjusting Setpoint

Contact Mode

1. Adjust the Setpoint to as low a value as possible using the cursor keys until the cantilever

pulls off the surface (and the Z-center voltage jumps to -220V).

2. Increase the Setpoint slightly until the cantilever begins to touch the surface again and an

image appears. Or, use the Force Calibration command to select the Setpoint and estimate

the contact force, as described in Chapter 17.

Note: The cantilever will typically adhere to the sample surface much less in ﬂuid;

therefore, it is often possible to image at much smaller contact forces in liquid

than in air.

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Dell 3100 Spezifikationen Seite 191